Tranmere's away trip to Salford City on New Year's Day has been postponed, the club has confirmed.
It is the second fixture the Birkenhead club has had to scrap over the Christmas period, after a Covid outbreak caused Rovers to postpone their trip to Hartlepool United on Wednesday, December 29.
The club was awaiting the results of PCR tests this morning, to determine whether the match on Saturday could go ahead.
A statement on the club website said: "The club has worked hard to ensure that it has been able to fulfil all fixtures. However, taking into account the injuries and positive COVID-19 cases with the squad, the club does not have a sufficient number of players available in order to fulfil the fixture.
"In light of the increased transmission rate of the Omicron variant, the EFL will now review the circumstances surrounding the postponement. In addition it will assist affected clubs throughout this challenging period to help them mitigate against the risk of infection amongst players and staff, while ensuring their obligations to the EFL regulations are maintained.
"A rearranged date for the fixture will be confirmed in due course."
It is not known how many of Tranmere's playing squad are vaccinated against Covid-19.
But when the Globe asked manager Micky Mellon what the club's policy on vaccination was, after the 1-0 win against Leyton Orient on December 18, he said: "You can't make people get vaccinated. That's not the way that this country works.
"You can only encourage them and tell them of situations that have happened with people who've not been vaccinated.
"I certainly have, because I believe that it's the right thing to do. But the players are entitled to their opinion of what they want to do. They're the chief executives of themselves.
"But I would certainly encourage everybody to get vaccinated and make sure that we see the last of this disease and hopefully we'll all get through it safely."
Tranmere currently sit third in League Two, occupying one of the automatic promotion places, having won their last five league matches.
Comments: Our rules
We want our comments to be a lively and valuable part of our community - a place where readers can debate and engage with the most important local issues. The ability to comment on our stories is a privilege, not a right, however, and that privilege may be withdrawn if it is abused or misused.
Please report any comments that break our rules.
Read the rules here