TRANMERE Rovers have confirmed that manager Gary Brabin has left the club with immediate effect.
The announcement comes a day after they were beaten 1-0 by Sutton United.
It was their third defeat in four games and followed a run of seven games unbeaten at the start of the campaign.
Club chairman Mark Palios said this morning: "Gary Brabin and I have agreed today that his position at Tranmere will end immediately.
"I would like to thank Gary for his hard work and contribution over the past 16 months in building a strong squad of players, getting us back fighting for promotion places and setting some club records in the process.
"However, we have agreed that it is best now to allow someone else to take the club to the next stage."
Tranmere's National League campaign continues on Saturday, against Woking at Prenton Park.
Rovers are currently fifth on the National League table, four points behind leaders Dagenham & Redbridge.
Brabin was named the VNL Manager of the Month in August.
He took charge of Rovers in May last year following the club's relegation from the Football League.
He took the club to sixth place in his first season, narrowly missing out on a play-off place.
This season began with high optimism, with six victories and one draw from the opening seven games.
He led the team to wins over Bromley, Barrow, Eastleigh, Boreham Wood, Maidstone United and Guiseley with a draw away at Southport.
Rovers will make a statemment regarding interim arrangements shortly.
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